|
KMID : 0545119970070010021
|
|
Journal of Microbiology and Biotechnology
1997 Volume.7 No. 1 p.21 ~ p.24
|
|
|
Investigation of the Relationship between Protein, Message and Inducer Concentrations in Recombinant E. coli Cells
|
|
LENE, JORGENSEN
THOMAS, CONNOR J./O´NEILL, BRIAN K./MIDDELBERG, ANTON P.J.
|
|
|
Abstract
|
|
|
|
Chloramphenicol acetyl transferase (CAT) protein and mRNA levels in E. coli were determined following induction of a tac::cat construct by isopropyl-β-thiogalactopyranoside (IPTG). High cat mRNA levels did not directly reflect CAT protein levels, in either shakeflask experiments or fermentations. Furthermore, concentrations of IPTG resulting in the highest levels of expression of cat mRNA, were different to those resulting in highest levels of CAT protein. The data suggest that high transcriptional activities lead to limitations at the translational level.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|